The Tele's pickups sound different than say a Stratocaster. The neck pickup is duller and perhaps more hollow. The '52 spec Teles have especially mellow sounding neck pickups to accomidate bass-like/rhythm tones of that era's music. I also find that Teles tend to have a slightly thicker bridge pickup but with more presense than a Strat bridge pickup. The middle selection on a Tele is less "clucky" and sounds totally different than the middle pickup on a Stratocaster. To me though, the biggest differences are the guitar's neck and bridge. Something about Telecasters make you want to play twangy riffs and do those country style bends. The bridge design allows for a lot of sustain being that it's mounted straight to the body. Don't take my word for it, try a bunch of both and see for yourself.
'67 and '74 Fender Twin Reverbs, '74 Marshall 1987 lead mkII, Metro Superlead 100. Pedals from TC Electronic, Ibanez, Dunlop, BK Butler, Electro-Harmonix, Fulltone, Maestro/Gibson, Loopmaster switching, VoodooLab, Boss. Gibson and Fender guitars, Dimarzio pickups.